Question 1

The amplification of recombinant plasmids occurs by:

Accepted Answer

A)   the growth of the plasmid DNA.
B)   DNA replication of plasmids outside a host cell.
C)   the process of growth and division of the host cell.
D)   transformation of bacterial cells.
E)   the polymerase chain reaction.F) B) and D)
G) B) and C)

Question 2

Explain how herbicide-tolerant weeds ("superweeds") could appear in areas nearby transgenic herbicide-tolerant crops. What issues arise with this scenario?

Accepted Answer

Transgenic herbicide-tolerant crops may ...

Question 3

List and briefly explain three properties of plasmids that allow them to be used as effective DNA cloning vectors.

Accepted Answer

Plasmids have an origin of rep...

Question 4

____ is a technique that can be used to separate DNA molecules on the basis of their size.

Accepted Answer

A)   Separation gradient
B)   Electrophoresis
C)   Selective sorting
D)   Cloning
E)   PCRF) A) and E)
G) D) and E)

Question 5

Transgenic salmon are genetically engineered to grow faster than their wild type counterparts. They do not grow larger; they simply reach their full size in shorter time. Transgenic salmon that are grown in outdoor pens are all sterile (non-reproducing) females. Why is this important?

Accepted Answer

If the transgenic fish escape from their...

Question 6

Clues to the structure and function of a gene can be gained through bioinformatics .

Accepted Answer

A) True 
 B)False

Question 7

Retroviruses make ____ by the process of ____.

Accepted Answer

A)   RNA copies of DNA; reverse transcription
B)   DNA copies of RNA; reverse transcription
C)   genetic probes; Southern blotting
D)   genetic probes; Northern blotting
E)   telomerase; mutagenesisF) A) and C)
G) A) and E)

Question 8

When RNAs separated by electrophoresis are transferred to a membrane and detected using a nucleic acid probe, the result is called a(n) Western Blot . __________________

Accepted Answer

A) True 
 B)False

Question 9

Match between columns
                        Premises: Step 4
Step 1
Step 2
Step 3
Step 5

Accepted Answer

The Answer of Load the DNA fragments on the gel is...

Question 10

Figure 15-1  Refer to the accompanying figure. The use of antibiotic medium at the end of this process:

Accepted Answer

A)   selects against plasmids containing human DNA fragments.
B)   selects for plasmids containing particular DNA fragments.
C)   selects for bacteria containing plasmids.
D)   selects for bacteria lacking plasmids.
E)   prevents contamination of the medium.F) A) and E)
G) A) and D)

Question 11

cDNA libraries contain:

Accepted Answer

A)   single stranded DNA.
B)   DNA synthesized with the use of DNA polymerase.
C)   DNA synthesized from and complementary to an mRNA template.
D)   introns and exons.
E)   genomic DNAF) A) and C)
G) A) and B)

Question 12

The total DNA in a cell is referred to as the:

Accepted Answer

A)   genome.
B)   cDNA.
C)   library.
D)   chromosomal DNA.
E)   Human Genome projectF) A) and D)
G) D) and E)

Question 13

Bacteria methylate their DNA in order to:

Accepted Answer

A)   prepare for DNA replication.
B)   prepare for mitosis.
C)   effectively infect other cells.
D)   protect it from restriction enzymes.
E)   prevent viral DNA from hybridizing with bacterial DNA.F) B) and E)
G) D) and E)

Question 14

The polymerase chain reaction was developed by:

Accepted Answer

A)   Francis Crick.
B)   Walter Gilbert.
C)   E.M. Southern.
D)   Kary Mullis.
E)   Leland Hartwell.F) A) and D)
G) A) and B)

Question 15

Why is it unlikely that genetically engineered strains of bacteria would pose a threat to the environment?

Accepted Answer

A)   Genetically engineered bacteria cannot survive exposure to UV radiation.
B)   Genetically engineered strains of bacteria are strictly regulated, making accidental release unlikely.
C)   Genetically engineered strains of bacteria cannot survive exposure to oxygen.
D)   Genetically engineered strains of bacteria are poor competitors of wild strains.
E)   Genetically engineered strains of bacteria require supplemental nutrients to survive.F) B) and C)
G) B) and E)

Question 16

Figure 15-1  What process or enzyme was used for Step 1 in the accompanying figure?

Accepted Answer

A)   PCR
B)   a bacteriophage
C)   a reverse transcriptase
D)   a DNA ligase
E)   a restriction enzymeF) A) and D)
G) A) and C)

Question 17

Bacteriophages are:

Accepted Answer

A)   viruses that infect bacteria.
B)   plasmids that infect bacteria.
C)   bacteria that infect other bacteria.
D)   enzymes that destroy bacteria.
E)   recombinant organisms.F) A) and D)
G) A) and E)

Question 18

Southern blotting is a process where ____ is transferred from a gel to a ____where a probe can be applied.

Accepted Answer

A)   DNA; water solution
B)   protein; water solution
C)   DNA; nitrocellulose filter
D)   RNA; nitrocellulose filter
E)   protein; nitrocellulose filterF) A) and E)
G) A) and D)

Question 19

Match between columns
                        Premises: Transgenic pest-resistant crops may kill all vulnerable individuals, creating a population of pesticide-resistant pests.
Transgenic lab animals, such as mice, are engineered to carry human genes, allowing for better study of human diseases.
Farmers who plant transgenic herbicide-tolerant crops tend to use excess herbicides.
Transgenic pest-resistant crops may affect non-target pests.
Transgenic papayas resistant to the ring spot virus saved the papaya industry in Hawai'i.
Growing transgenic pest-resistant crops means less pesticide spraying.
All transgenic salmon are sterile females; therefore, they cannot breed with wild salmon populations.
Gene flow from transgenic herbicide-tolerant plants may create herbicide-tolerant weeds.
Transgenic salmon that grow faster than wild salmon may result in less pollution from salmon farms.
Transgenic animals, such as sheep and cows, produce medicinal proteins in their milk. This creates a larger and cheaper supply of some human drugs.

Accepted Answer

The Answer of Advantage and Disadvantage is...

Question 20

To avoid the introduction of introns into the vector, a ____ copy of mature mRNA is made, using the enzyme ____.

Accepted Answer

A)   sDNA; RNA polymerase
B)   cDNA; DNA ligase
C)   cDNA; reverse transcriptase
D)   sDNA; reverse transcriptase
E)   cDNA; DNA polymeraseF) C) and D)
G) A) and D)

Exam 15: Dna Technology and Genomics

____ is a technique that can be used to separate DNA molecules on the basis of their size.

Correct Answer
verified

Explain how herbicide-tolerant weeds ("superweeds") could appear in areas nearby transgenic herbicide-tolerant crops. What issues arise with this scenario?

Correct Answer
verified
Transgenic herbicide-tolerant crops may ...
View Answer

Clues to the structure and function of a gene can be gained through bioinformatics .

Correct Answer
verified

To avoid the introduction of introns into the vector, a copy of mature mRNA is made, using the enzyme .

Correct Answer
verified

Bacteria methylate their DNA in order to:

Correct Answer
verified

When RNAs separated by electrophoresis are transferred to a membrane and detected using a nucleic acid probe, the result is called a(n) Western Blot . __________________

Correct Answer
verified

The amplification of recombinant plasmids occurs by:

Correct Answer
verified

Figure 15-1 What process or enzyme was used for Step 1 in the accompanying figure?

Correct Answer
verified

Retroviruses make by the process of .

Correct Answer
verified

List and briefly explain three properties of plasmids that allow them to be used as effective DNA cloning vectors.

Correct Answer
verified
Plasmids have an origin of rep...
View Answer

cDNA libraries contain:

Correct Answer
verified

Determine whether each scenario describes an advantage or disadvantage of transgenic organisms.

Match the sequence of the steps for the Southern Blot assay.

The total DNA in a cell is referred to as the:

Correct Answer
verified

Transgenic salmon are genetically engineered to grow faster than their wild type counterparts. They do not grow larger; they simply reach their full size in shorter time. Transgenic salmon that are grown in outdoor pens are all sterile (non-reproducing) females. Why is this important?

Correct Answer
verified
If the transgenic fish escape from their...
View Answer

Figure 15-1 Refer to the accompanying figure. The use of antibiotic medium at the end of this process:

Correct Answer
verified

Southern blotting is a process where is transferred from a gel to a where a probe can be applied.

Correct Answer
verified

The polymerase chain reaction was developed by:

Correct Answer
verified

Why is it unlikely that genetically engineered strains of bacteria would pose a threat to the environment?

Correct Answer
verified

Bacteriophages are:

Correct Answer
verified

Exam 15: Dna Technology and Genomics

____ is a technique that can be used to separate DNA molecules on the basis of their size.

Correct AnswerverifiedShow Answer

Explain how herbicide-tolerant weeds ("superweeds") could appear in areas nearby transgenic herbicide-tolerant crops. What issues arise with this scenario?

Correct AnswerverifiedTransgenic herbicide-tolerant crops may ...View AnswerShow Answer

Clues to the structure and function of a gene can be gained through bioinformatics .

Correct AnswerverifiedShow Answer

To avoid the introduction of introns into the vector, a ____ copy of mature mRNA is made, using the enzyme ____.

Correct AnswerverifiedShow Answer

Bacteria methylate their DNA in order to:

Correct AnswerverifiedShow Answer

When RNAs separated by electrophoresis are transferred to a membrane and detected using a nucleic acid probe, the result is called a(n) Western Blot . __________________

Correct AnswerverifiedShow Answer

The amplification of recombinant plasmids occurs by:

Correct AnswerverifiedShow Answer

Figure 15-1 What process or enzyme was used for Step 1 in the accompanying figure?

Correct AnswerverifiedShow Answer

Retroviruses make ____ by the process of ____.

Correct AnswerverifiedShow Answer

List and briefly explain three properties of plasmids that allow them to be used as effective DNA cloning vectors.

Correct AnswerverifiedPlasmids have an origin of rep...View AnswerShow Answer

cDNA libraries contain:

Correct AnswerverifiedShow Answer

Determine whether each scenario describes an advantage or disadvantage of transgenic organisms.

Match the sequence of the steps for the Southern Blot assay.

The total DNA in a cell is referred to as the:

Correct AnswerverifiedShow Answer

Transgenic salmon are genetically engineered to grow faster than their wild type counterparts. They do not grow larger; they simply reach their full size in shorter time. Transgenic salmon that are grown in outdoor pens are all sterile (non-reproducing) females. Why is this important?

Correct AnswerverifiedIf the transgenic fish escape from their...View AnswerShow Answer

Figure 15-1 Refer to the accompanying figure. The use of antibiotic medium at the end of this process:

Correct AnswerverifiedShow Answer

Southern blotting is a process where ____ is transferred from a gel to a ____where a probe can be applied.

Correct AnswerverifiedShow Answer

The polymerase chain reaction was developed by:

Correct AnswerverifiedShow Answer

Why is it unlikely that genetically engineered strains of bacteria would pose a threat to the environment?

Correct AnswerverifiedShow Answer

Bacteriophages are:

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified
Transgenic herbicide-tolerant crops may ...
View Answer

Correct Answer
verified

To avoid the introduction of introns into the vector, a copy of mature mRNA is made, using the enzyme .

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Retroviruses make by the process of .

Correct Answer
verified

Correct Answer
verified
Plasmids have an origin of rep...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
If the transgenic fish escape from their...
View Answer

Correct Answer
verified

Southern blotting is a process where is transferred from a gel to a where a probe can be applied.

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified